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Figure 5: RNA abundance evaluated by polymerase chain reaction. Three strains of ovine dermal papilla cells (red, green, and blue lines) were compared in duplicate (circles, squares) at different stages of aggregation (subconfluent, SC; early aggregates, EA; late aggregates, LA). Expression was normalized to GAPDH and it is presented on a log-scale. Values within each duplicate were standardized to a mean of 1.0, allowing the duplicates to be plotted on a common scale. Thus, these data represent the relative changes in marker abundance with aggregation state. (a): versican V0. (b): versican V1. (c): versican V2. (d): versican V3. (e): Transforming growth factor-β1. (f): wif-1. (g): Neural cell adhesion molecule. (h): corin, (i): hey1. Asterisks denote statistically significant differences at P < 0.05 |
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